The second installment of the Demon Slayer stage adaptations; it ran from August 7th-August 31st 2021 in Tokyo and Osaka.

The official stage play adaptation of Koyoharu Gotoge's series Kimetsu no Yaiba. The time is Taisho, Japan. One day, Tanjiro, a kind-hearted boy who sells charcoal, has his entire family killed by a demon. Furthermore, his only surviving sister, Nezuko, has been transformed into a demon. Tanjiro is devastated by the hopeless reality, but decides to pursue the path of "demon hunting" in order to return his sister to a human and avenge the demon that killed his family. The sad story of brother and sister, woven together by humans and demons, begins now!

Having passed the tryouts (selection exams), rookie recruits Akira, Will, Ren, and Gast proudly become HELIOS's 13th generation of Heroes. They attend the entrance ceremony with their own thoughts in mind. However, the stage for the Heroes' festive entrance ceremony is also a golden opportunity for Siams and Shin of Eclipse, who are after Substance, to set their sights on HELIOS. Eclipse appears before the HELIOS Heroes in a huge explosion. Thanks to the efforts of Akira and Will's mentors, Brad and Oscar, they manage to defeat Eclipse and avoid disaster. Meanwhile, Akira, who didn't perform as well as he'd hoped, seems to vent his frustration by boasting that he'll make South Sector, where he's been assigned, number one in LOM (League of Millions), a ranking competition for Heroes. Akira and the others are excited about being able to act as heroes, but Eclipse, operating in secret, is plotting to steal the Substance from HELIOS...

[HELIOS] It's been a while since the 13th training team began... The North Sector, which boasted an undisputed No. 1 ranking in the [Sector Rankings], finds itself in an unexpected predicament. Marion, the youngest mentor in history, is deeply hurt by North's drop in ranking, and is unable to control his emotions, becoming frustrated. The other mentor, Victor, is only interested in researching [Substance]. While rookie Gast struggles to rebuild the team, fellow rookie Ren is obsessed with revenge against [Eclipse], who killed his sister and parents. The team is far from united. As if reflecting the team's fragmented situation, the city's security deteriorates, and trouble breaks out one after another. To make matters worse, an [Eclipse] entity known as "Zero" begins appearing in the North as well...

Based on the in-game event Special Investigation: Tenpou Edo.