A girl from a peaceful, Edo-era Japanese village seeks revenge for the death of a disgraced elder with the help of her sister and a lethal lady ninja nun. It's the time of the samurai and ninja, and Shogun Yoshimune rules Japan. Beneath the idyllic façade of the quant villages populating the countryside dwells a twisted sexual underbelly that affects the wealthy and impoverished alike. When one of the village elders commits seppuku after being raped, Kaede and her sister decide to take justice into their own hands and track down the perpetrator. Aided by a nun who believes in Old Testament-style retribution, the two sisters embark on a bloody mission of vengeance. Along the way, the three women slice and dice their way through lies and secrets while discovering that the story behind the rape is much more complex than any of them suspected.

The story revolves around a man named Takuma who works under the legendary samurai Sanada Yukimura teaching a secret technique called “Kakure Musou”. However, Sanada won't allow Takuma to actually fight for him because he's hampered by a painful disease of the eyes. It's the worst kind of humiliation for a warrior like Takuma. A woman, played by Asami (Sukeban Boy, The Machine Girl), eventually makes a special medicine that helps him recover from his affliction. Meanwhile, Kasumi is sent to investigate a bandit who intends to assassinate Sanada. As the threat of assassination draws closer the shocking truth is exposed and Kasumi is forced to face off against an unlikely betrayer.

It's been two years since Kasumi chose the Kunoichi lifestyle and she now has a new enemy in Hattori Hanzo. Hanzo's wish is to penetrate the Ueda castle. So he enlists his own Kunoichi to seduce the gatekeeper, probing him for security loopholes.