The special is hosted by Tony Danza and Annie Potts celebrating 50 years of William Hanna and Joseph Barbera's partnership in animation. This is the first animated project to be broadcast in Dolby Surround sound system.

This time, the rivals team up to help a cowgirl and her brother save their homestead from a greedy land-grabber, and they’re going to need some help! Jerry’s three precocious nephews are all ready for action, and Tom is rounding up a posse of prairie dogs. But can a ragtag band of varmints defeat a deceitful desperado determined to deceive a damsel in distress? No matter what happens with Tom and Jerry in the saddle, it’ll be a rootin’ tootin’ good time!

Tom fights with another cat over Jerry.

Tom invites Toots to an elegant dinner. However, he's made the mistake of trying to put Jerry to work, as a serving boy, a corkscrew, and other tasks. Jerry puts up with a little of this, but mostly gets revenge on Tom.

Tom calls the exterminators, but they send a cat, who despite his various tools, doesn't fare much better than Tom usually does.

Tom's advances on a young jive-talking girl cat get nowhere; nowhere, that is, until Tom gets a zoot suit. Armed with his miles of fabric and a new cool lingo, Tom still has to deal with the tricks of his nemesis, Jerry.

Tom hears a ghost story on the radio and is spooked by it; Jerry notices this and takes advantage of it, using a variety of tricks to scare Tom.

Tom's cousin George, who's terribly afraid of mice, comes to visit. Jerry's confused, since Tom and George look alike.

Dueling cat-and-mouse team Tom and Jerry hit the high seas on a hunt for buried treasure in this pirate adventure. The tale begins when crew member Tom sets sail with an infamous pirate and finds a treasure map along with stowaway Jerry. The furry swashbucklers race to a deserted island where X marks the spot, but along with battling each other, they must outwit ruthless buccaneers, angry monkeys and a giant octopus to strike it rich.

Tom ties up Spike and sneaks into the courtyard of the glamorous Toodles Galore with his bass, hoping to woo her with his song, much to the annoyance of a sleeping Jerry.