A beautiful school is about to reopen after many years and Luì and Sofì (Me against You) are the special guests of the inauguration party, their friend Pongo awaits them there. The school, however, could hide a mystery and, once again, the Me against You will have to face the evil Mr. S with courage and try to sabotage his evil plans in the name of friendship. In the school, for the first time, Luì and Sofì could learn an important secret about their past. A new “magical” adventure in the cinema for Luì and Sofì, in a world of magic, with lots of surprises and fun for their little fans and all families.

Luì is about to receive his diploma as a scientist until Mr. S and Perfidia will try to undermine the Me against You.

Bologna, 1954. Taddeo, a young man of 18 whom everyone calls Kid, dreams of becoming one of the regulars of the mythical Margherita Café, located under the portico across from his family's home. Through a ploy, he manages to get work as the chauffeur for Al, the neighborhood's most glamorous and mysterious resident. Having been taken under Al's wing, Taddeo gets to witness the adventures of Bep, who is in love with Marcella the entraineuse; the trials and tribulations of Gian, an aspiring singer and the victim of a horrible practical joke; the crazy behavior of Manuelo, a small-time robber with a sex phobia; the meanness of Zanchi, inventor of the elastic necktie; and the bizarre manias of Sarti, a ballroom dancing champion who wears his tux day and night. And Taddeo's home life is no less out of the ordinary, considering his mother is being led on by the family physician while his grandfather has fallen head over heels for a well-built piano teacher.

After their adventures in Ancient Egypt, Luì and Sofì just want to rest, but a new threat looms over the Earth. A magical spring hidden in the jungle holds the key to undoing all the world's spells, but if tainted, it can turn the Earth into a barren, lifeless wasteland. Viperiana has discovered this and with the help of her faithful Serpe servant has already started mixing enchanted potions in the spring that turn the water purple; it will only take a few days for everything to be irreversibly poisoned. A fight against time begins for Luì and Sofì who, together with Pongo's assistant and accompanied by the guide Serenino and Tara, daughter of the Pesantosi tribal chief, will have to decipher the map that will lead them to the magical spring and foil Viperiana's diabolical plan . But they have to be careful, because an unexpected enemy lurks among them.

The alliance of Malefics, formed by Mr. S., Perfidia, Viperiana and Serpe have convinced the world that He and Sophi are criminals and the two, in order not to be wanted, join other characters to prove their innocence and bring harmony back to the planet.