Lupin's plans to intercept two old glass photographic plates from being delivered to a mysterious art dealer named Mr. Suzuki misfires, and it doesn't help matters that Goemon and Jigen are suffering from... efficiency problems. Meanwhile, Inspector Zenigata has a new tag-along in the Lupin chase: the beautiful young reporter Maria. But what is Maria's connection to the mysterious Mr. Suzuki?

With the Gyaos re-emerging, Gamera's ties to humanity have been severed with his bond to Asagi broken. Nagamine and Asagi investigate while an orphaned girl named Ayana discovers a new creature she names Iris. Nagamine and Asagi must reach Ayana before she takes her revenge on Gamera, who she blames for the death of her family.