Jane and Jackie are driven far beyond the brink of madness after witnessing the brutal rape and murder of their younger sister by a group of marauding college fraternity brothers. Upon the release from an insane asylum, the sisters embark on a relentless search for the guys that raped and strangled their sister. Obsessed with revenge and fueled by a hatred of all men, they kill any man that crosses their path, retaining his severed genitals as a trophy.

Mean and domineering Jane and the sweet and ditsy Jackie are two radically contrasting sisters who suffer from severe trauma after witnessing the murder/suicide of their parents as well as seeing their other sibling Janice raped and killed by her assailants. The pair are deemed fit to re-enter society and get released from a sanitarium where they have undergone extensive rehabilitation therapy. However, Jane and Jackie prove to be anything but sane and harmless as they embark on a vicious spree in which they torture, murder, and mutilate any man luckless enough to cross their lethal paths.