Eve's Daughter was the fourth and final screen teaming of Billie Burke and Thomas Meighan. This time out, Burke is cast as Irene Simpson-Bates, who is disheartened to learn that her millionaire father has left her with a mere $15,000. She is subsequently courted by two men: Poor but respectable John Norton (Thomas Meighan), and rich but disreputable Courtenay Urquahrt (Lionel Atwill, in his American screen debut).

Four sisters come of age in America in the aftermath of the Civil War.

Youth meets Ambition and leaves Love, his mother, and his small-town roots for the big city. There, in his search for Experience, he meets Pleasure and hangs out at the Primrose Path with the likes of Temptation and Intoxication. Back home, Youth's mother dies, and Love tries unsuccessfully to reach him. When Youth's money runs out his newfound friends all leave him and he sinks into a life of drug addiction, aided by Habit.

Alice Lindsay arrives in New York from a small town and becomes part of Greenwich Village Bohemian life. Alice resists the advances of Gwenne Stevens, an advocate of free love, and marries civil engineer Samson Rathbone

A daughter of the slums has a little brother and makes a brave attempt to earn enough for both herself and Jimmy after her father is sent to jail on a fake charge trumped-up by "Nifty" Mendez, who is very anxious to get her in his toils. Betty escapes from the city and Nifty. On the road Jimmy is struck by Rodney Channing's motor. The injury is not serious but Jimmy must remain in Channing's home for some time. During the visit, love grows up between Channing and Betty, who is now of course very happy. But a newspaper announcement and the author send Nifty again into her life and simultaneously with his entrance comes the exit of happiness.