Barney and his friends take the stage to delight and entertain everyone at Radio City Music Hall. They all work together to make a special surprise for the audience using items from "The Barney Bag". But before they are able to share it, a new visitor, The Winkster, sneaks away with the bag.

Barney the Dinosaur joins his friends on an imaginative trip to school, where they explore classrooms, learn about safety, play games, and discover the fun of learning together. With songs, storytelling, and gentle lessons, Barney Goes to School encourages preschool-aged viewers to feel confident and excited about starting school.