While trailing Forest Ranger Charles Carter, who is suspected of permitting lumber man Henry Mitchell to cut restricted timber, Gene fires at a dangerous mountain lion and apparently kills Carter. Actually, Bill Wright, Mitchell's associate, killed Carter because the ranger had discovered tussock moth infestation in the forest, and if the infestation was not reported, the trees would die and have to be cut, thereby profiting Mitchell and Wright. In order to compensate the best he can, Gene sells his sportsman's camp and gives the money to Carter's daughter Helen . En route to Texas, Gene discovers the infestation and is assigned by the Forest Department to supervise the program of spraying the area with DDT from the air. After the first day of spraying, the DDT is blamed by furious stock men for the many animals found dead of poisoning.

Ranch owner Sandra, fresh from animal husbandry school, brings a flock of sheep into cattle country. The local ranchers don't like it, and ranch foreman Gene must deal with it.

Gene Autry hunts bank robbers Al Bartlett and Trot Lucas with his old friend Mike. Bartlett, to throw off his pursuers, kills Trot and his own brother. When Kitty Bartlett comes to town claiming to be the slain Bartlett's widow, Gene has to save her from the irate townspeople who are not aware that her name isn't Bartlett but she really is the daughter of a law officer slain by Al Bartlett. Ben Luder, a local hood, tricks Bartlett back into town by saying he has to fixed to have Doc Larry Taylor do plastic surgery on him. En route they meet Doc and his assistant Helen Ellis and Ben's ruse is exposed. Bartlett kills Ben and forces Doc to drive him to the railroad. Gene, in a fight atop a runaway train, captures Bartlett.

At the Texas Centennial in Dallas Autry confuses two girls by being himself and his own stunt double. When cowboy star Tom Ford disappears, Wilson gets his double Gene Autry to impersonate him. But Ford owes gangster Rico $10,000 and Rico arrives to collect. He fails to get the money but learns that Autry is an impersonator and now blackmails Wilson and his movie studio. Original version runs 71 minutes, edited version runs 59 minutes.

As the sheriff of a small western town, Autry sings his way into a relationship with Eleanor, a singer from a Chicago nightclub who earlier witnessed a murder.

A criminal gang provokes the local Apaches in order to divert the authorities' attention from their own activities.

An outcast gambler hijacks a wagon train of eligible women taken west by a mayor.

A singing territorial ranger (Gene Autry) spots his younger brother in an outlaw gang.

Gene is hired to be foreman of the Big Sombrero ranch by Jim Garland, who is handling all the business affairs of the owner, Estrellita Estrada, who is more interested in going to America than taking care of her Mexican holdings. Gene, discovering Garland's plan to run all the Mexican rancheros off the ranch, turns against his boss and shortly finds himself in the middle of cattle stampedes and an avalanche started by Garland's men.

A rancher tries to convince an Indian tribe to relocate so their land can be used to provide water for Kansas City.